Technical Data
Stromal Cell Derived Factor 1alpha, Recombinant, Human (SDF1a)
Molecular Biology Storage: -20CShipping: Blue Ice
Chemokine SDF-1 is highly conserved among species and belongs to a large family of small, structurally related cytokines. It is a chemoattractant for T lymphocytes, monocytes, and hemopoietic stem cells and is constitutively expressed in bone-marrow-derived stromal cells. Recent reports indicate that SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. SDF-1a and SDF-1b, are generated by alternative RNA splicing of transcripts encoded by a single gene. SDF-1a differs from SDF-1b by four C-terminal amino acid residues absent in SDF-1a. As yet, no functional differences have been reported.
Stromal Cell-Derived Factor-1 (SDF1 ) is a recently discovered protein belonging to the -chemokine (C-X-C) family of cytokines. Human SDF1 is an 8kD protein containing 68 amino acid residues.
Recombinant Human SDF-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8008 Dalton. The rHuSDF-1 is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu

Biological Activity:
The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% by RP-HPLC and SDS-PAGE. Chromatographically purified.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder without additives.
Reconsitute with sterile ddH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Caveolin plays a central role in endothelial progenitor cell mobilization and homing in SDF-1-driven postischemic vasculogenesis. Circ Res (2006) 98:1219-1227. 2. Effects of CCR5-delta32, CCR2-64I and SDF-1-3'A polymorphic alleles on human immunodeficiency virus 1 (HIV-1) infection in the Polish population, Wiad Lek (2005) 58:500-507. 3. HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1. BMC Biochem (2006)7:2. 4. An SDF-1 trap for myeloid cells stimulates angiogenesis. Cell (2006) 124:18-21. 5. Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing. Bone (2006) 38:497-508. 6. The SDF-1/CXCR4 pathway and the development of the cerebellar system. Eur J Neurosci (2005) 22:1831-1839.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.