Technical Data
S7975-51B
Stromal Cell Derived Factor 1 alpha, Recombinant, Mouse (SDF1a)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 68aa from mouse Stromal Cell Derived Factor 1 alpha expressed in E. coli.

Molecular Weight:
~7.9kD

Biological Activity:
Determined measuring dose-dependent chemotaxis with human peripheral blood monocytes. A concentration range of 50-100ng/ml produced significant chemotaxis. SDF-1 is a CXC chemokine that binds to the chemokine receptor CXCR4. SDF-1 is widely expressed by cells of most tissues, including bone marrow stromal cells. SDF-1 is a chemoattractant for mononuclear cells and CD34+ hematopoietic progenitor cells.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~7.9kD
Purity: ~99% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder in 10 mM acetic acid, carrier free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Zlotnik, A., J. Morales, and J.A. Hedrick (1999) Recent advances in chemokines and chemokine receptors. Crit. Rev. Immunol. 19:1-47.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.