Technical Data
S7975-61
Stromal Cell Derived Factor 1beta, aa1-72, Recombinant, Human (SDF1b, CXCL12b, Pre-B Cell Growth-stimulating Factor, PBSF, hIRH, Chemokine (C-X-C Motif) Ligand 12b, TPAR1, SCYB12, TLSF-b)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
Chemokine SDF-1 is highly conserved among species and belongs to a large family of small, structurally related cytokines. It is a chemoattractant for T lymphocytes, monocytes, and hemopoietic stem cells and is constitutively expressed in bone-marrow-derived stromal cells. Recent reports indicate that SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. SDF-1a and SDF-1b, are generated by alternative RNA splicing of transcripts encoded by a single gene. SDF-1a differs from SDF-1b by four C-terminal amino acid residues absent in SDF-1a. As yet, no functional differences have been reported.

Stromal Cell-Derived Factor-1beta (SDF1b) is a recently discovered protein belonging to the a-chemokine (C-X-C) family of cytokines. Human SDF1b is an 8.5kD protein containing 72 amino acid residues.

Source: E. coli

Form: Lyophilized protein without additives.

Stability: Lyophilized SDF1b, though stable at room temperature, is best stored desiccated below 0C. Reconstituted human SDF1b should be stored in working aliquots at -20C.

Purity: 98% by SDS-PAGE and HPLC analyses.

Endotoxin: 0.1ng/ug

Reconstitution: Lyophilized SDF1b is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Biological Activity: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 20-80ng/ml.

Concentration Range for most in vitro applications: 0.1-1mg/ml

AA Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM

Country of Origin: USA

Molecular Weight:
8.5kD
Source: Human

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.