Technical Data
S7975-65
Stromal cell-derived growth factor, 33-173aa, Recombinant Human (IL-25, IL27, IL27w, Interleukin-25, Protein C19orf10 precursor, R33729_1, SF20, Stromal cell-derived growth factor SF20, Uncharacterized protein C19orf10 precursor)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
SF20/25, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. Recombinant human SF20/IL25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.

Source:
Human, His-tagged, Recombinant, E. coli, 33-173aa

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18kD
Source: E. coli
Purity: Purified: 95% by SDS-PAGE.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mm Tris-HCl buffer, pH 8.0, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Weiler T., et al. (2007) Arthritis Res Ther. 9, R30. 2. Tulin E.E., et al. (2001) J. Immunol. 167 (11): 6338-47

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.