		Technical Data
S7980-01B	SMC1, phosphorylated (Ser957) (Structural Maintenance of Chromosomes Protein 1A, Structural Maintenance of Chromosomes 1 Like 1, SMC1, SMC Protein 1A, SMC-1A, SMC-1-alpha, Sb1.8, SMC1A, DXS423E, KIAA0178, SB1.8, SMC1L1)

Description:
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.

Applications:
Suitable for use in ELISA, Dot Blot, Immunohistochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Dot Blot: 1:500
Immunohistochemistry: 1:50-100
Optimal dilutions to be determined by the researcher.

Control Peptide: S7980-01B1, SMC1, phosphorylated (Ser957), Control Peptide, Human (Structural Maintenance of Chromosomes Protein 1A, Structural Maintenance of Chromosomes 1 Like 1, SMC1, SMC Protein 1A, SMC-1A, SMC-1-alpha, Sb1.8, SMC1A, DXS423E, KIAA0178, SB1.8, SMC1L1)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Phosphorylated synthetic peptide corresponding to a region within human SMC1 (locus link ID 5884) around serine 957.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human structural maintenance of chromosomes-1 (SMC1) when phosphorylated at Ser957.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yazdi, P.T., et al., Genes Dev. 16(5):571-582 (2002). 2. Gregson, H.C., et al., Chromosome Res. 10(4):267-277 (2002). 3. Gregson, H.C., et al., J. Biol. Chem. 276(50):47575-47582 (2001). 4. Sumara, I., et al., J. Cell Biol. 151(4):749-762 (2000). 5. Schmiesing, J.A., et al., Proc. Natl. Acad. Sci. U.S.A. 95(22):12906-12911 (1998).