Technical Data
SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1)
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2).

Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:200
Western Blot: 1:2000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues surrounding Arg132 of human SMC3.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total human SMC3. Species Crossreactivity: Bovine, chicken, monkey, mouse, rat, xenopus and zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Peters, J.M. et al. (2008) Genes Dev 22, 3089-114. 2.Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35. 3.Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67. 4.Hauf, S. et al. (2005) PLoS Biol 3, e69. 5.Hauf, S. et al. (2001) Science 293, 1320-3. 6.Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77. 7.Chen, F. et al. (2002) J Biol Chem 277, 16775-81.