Technical Data
Substance P (NK, Neurokinin)
Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.

Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml. Detects Substance P in paraffin-embedded human spinal cord tissue sections when used with the appropriate secondary reagents.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG17H63Affinity Purified
100ug4C (-20C Glycerol)Blue IceHumanMouse
KLH-conjugate of Substance P, (RPKPQQFFGLM).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Recognizes human Substance P. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.