Technical Data
S8000-02D
Substance P (NK, Neurokinin)
Description:
Substance P is a tachykinin family neuropeptide that functions as a neurotransmitter and neuromodulator. In the CNS, Substance P is involved in the regulation of mood, anxiety, sleep, saiety, reinforcement, respiratory rhythm and pain processing. In nociception, the neuropeptide is released at local spinal cord synapse. Recent research now shows substance P is potentially involved in endothelial cell differentiation as well as diverse roles in neuroinflammatory response. The endogenous receptor for Substance P is neurokinin 1 receptor (NK1 receptor), which belongs to the tachykinin receptor sub-family of GPCRs.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:400-1:800 using Immunoflourescence. Incubate overnight at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Cryostat sections of rat colon

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceGuinea pig
Concentration:
Not determined
Immunogen:
Substance P conjugated to BSA.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile ddH2O. Dilute further in 0.1M PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes Substance P. Species Crossreactivity: Rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Witt, M. et al. Innervation of developing human taste buds. An immunohistochemical study. Histochem. Cell Biol. 1998, 109(3):281-291