Technical Data
Substance P (NK, Neurokinin)
Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. It stimulates smooth muscle contraction, gives rise to vasodilation and is involved in sensory functions. Substance P-containing tumors arising in the ileum are often associated with the carcinoid syndrome, characterized by flushing of the skin, diarrhea, bronchoconstriction and sudden drops in blood pressure. Substance P is commonly found in the midgut carcinoids and some of the symptoms may be related to this peptide.

Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin and frozen sections): 1:400-1:800 using immunofluorescence microscopy, with overnight incubation at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Frozen sections of rat colon.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul4C (-20C Glycerol)Blue IceRabbit
Not determined
Substance P conjugated to BSA
Supplied as a lyophilized powder. Reconstitute with 50-100 ul ddH2O or 40-50% glycerol for long-term storage. Dilute further in 0.1 M PBS with 1% BSA and 0.09% sodium azide.
Recognizes Substance P. Absorption with 10-100ug SP and NKA per ml diluted antiserum abolishes the staining while Neurokinin A does not. Species Crossreactivity: Human, porcine, rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hardebo JE et al, Neuroscience letters 101: 39-45 (1989). 2. Hauser JE et al., J Andrology 15(6): 510-520 (1994). 3. El-Salhy M et al, Scand J Gastroenterol 33: 394-400 (1998).