Technical Data
Substance P (NK, Neurokinin)
Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. It stimulates smooth muscle contraction, gives rise to vasodilation and is involved in sensory functions. Substance P-containing tumors arising in the ileum are often associated with the carcinoid syndrome, characterized by flushing of the skin, diarrhea, bronchoconstriction and sudden drops in blood pressure. Substance P is commonly found in the midgut carcinoids and some of the symptoms may be related to this peptide.

Sutiable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Immunofluorescence: 1:400-1:800 with overnight incubation at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Frozen sections of rat colon

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul-20CBlue IceGuinea pig
Not Determined
Substance P conjugated to BSA.
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O. Dilute further with PBS, 1% BSA, 0.09% sodium azide.
Recognizes Substance P. Absorption with 10-100ug SP and NKA per ml diluted antiserum abolishes the staining while GRP and NKB do not. Species Crossreactivity: human, rat, rabbit and fish (cod).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hardebo JE et al. Origins of substance P- and calcitonin gene-related peptide-containing nerves in the internal carotid artery of rat. Neuroscience letters 101:39-45 (1989) 2. Witt M et al. Innervation of developing human taste buds. An immunihistochemical study. Histochem. Cell Bio 109 (3), 281-291 (1998) 3. Shabazi F et al: Primary structure, distribution, and effects on motility of CGRP in the intestine of the cod Gadus morhua. Am J Physiol 44, R19-R44 (1998). 4. Glfert F et al: Histochem J 30, 435-445 (1998). 5. Kressel M et al: Anterograde tracing and immunohistochemical characterization of potentially mechanosensitive vagal afferents in the esophagus. J Comp Neurol 412, 161-172 (1999).