		Technical Data
S8000-05D	Substance P, aa4-11 (NK, Neurokinin)

Description:
Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. It stimulates smooth muscle contraction, gives rise to vasodilation and is involved in sensory functions. Substance P-containing tumors arising in the ileum are
often associated with the carcinoid syndrome, characterized by flushing of the skin, diarrhea, bronchoconstriction and sudden drops in blood pressure. Substance P is commonly found in the midgut carcinoids and some of the symptoms may be related to this peptide.

Applications:
Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue, no proteolytic treatment required).

Recommended Dilution:
Immunohistochemistry: 1:400-1:800 using PAP, with overnight incubation at 2-8°C.

Positive Control:
Frozen sections of rat colon.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice		Rabbit

Concentration:

Not determined

Immunogen:

Synthetic Substance P 4-11, conjugated to thyroglobulin using CDI.

Purity:

Serum

Form

Supplied as a liquid.

Specificity:

Recognizes Substance P. Absorption with 10-100ug SP 1-4 per ml diluted antiserum abolishes the staining. Species Crossreactivity: Human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Hardebo JE et al. Origins of substance P- and calcitonin gene-related peptide-containing nerves in the internal carotid artery of rat. Neuroscience letters 101:39-45 (1989)