Technical Data
S8000-12A
Substance P (NKA, NKNA, TAC2)
Description:
The COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:100-1:200
Immunohistochemistry (paraffin): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1/1-Ag 4-1 myeloma cells with spleen cells from Wistar rat.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10F477Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceRat
Concentration:
Not determined
Immunogen:
Substance P (BSA).
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% thiomersal.
Specificity:
Recognizes the COOH terminal end of substance P. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cuello, A.C. et al. (1980) Use of monoclonal antibodies in immunohistochemistry with special reference to the central nervous system. Brain Res. Bull. 5: 575-587. 2. Pioro, E.P. et al. (1985) Loss of substance P immunoreactivity in the nucleus of the spinal trigeminal tract after intradural tumour compression of the trigeminal nerve. Neurosci Lett. 58: 7-12. 3. Mai, J.K. et al. (1986) Substance P in the human brain. Neuroscience 17: 709-739. 4. Cuello, A.C. et al. (1979) Detection of substance P in the central nervous system by a monolconal antibody. Proc. Natl. Acad. Sci. USA 76: 3532-3536.