Technical Data
Substance P Receptor (NK1 Receptor, Neurokinin Receptor)
The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.
Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 (ECL) A band is seen at approximately 53kD representing human NK-1. The actual molecular weight may vary depending on the tissue used.
Optimal dilution determined by the researcher.

Positive Control:
SF9 whole cell membrane extracts

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul4C (-20C Glycerol)Blue IceHumanRabbit
A peptide derived from the N-terminus of human NK-1 sequence (KLH). Cellular localization: Cell membrane.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Recognizes human NK-1. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vigna, S.R., et al, The Journal of Neuroscience. 14(2): 834-845, 1994. 2. Mantyh, P.W., et al,. Science. 278: 275-279, 1997. 3. Mantyh, C.R. et al, . Gastroenterology. 111:1272-1280, 1996. 4. Mantyh, P.W. et al, PNAS. 92: 2622-2626, 1995. 5. Mantyh, P.W., et al, Science. 268: 1629-1632, 1995. 6. Mantyh, P.W. et al, Brain Research. 719: 8-13, 1996. 7. Allen, B.J., et al, . Journal of Neuroscience. 17(15): 5921-5927, 1997.