Technical Data
S8000-52
Substance P Receptor (NK1 Receptor, Neurokinin Receptor)
Description:
The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.

Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen 1:500-1:1000
Flow Cytometry: 1:3000
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A peptide derived from the human NK-1 amino acids with a cysteine added for conjugation to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS with 0.05% sodium azide.
Specificity:
Recognizes human Substance P Receptor (NK1 Receptor, Neurokinin Receptor). Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.