Technical Data
S8060-09B
Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)
Description:
Superoxide dismutase (SOD) is an endogenously produced
intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extracellular space (9). SOD3 can also be distinguished by its heparin-binding capacity (1).

Applications:
Suitable for use in Western Blot, Immunoprecipition, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot (Colorimetric): 0.2ug/ml
Immunoprecipitation: 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Human Cu/Zn SOD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide, 50% glycerol.
Specificity:
Recognizes human Cu/Zn superoxide dismutase (SOD) at ~23kD. Species crosseactivity: monkey, mouse, rat, bovine, coral, canine, hamster, porcine, rabbit, sheep and Xenopus at ~19kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kurobe, N., and Kato, K. (1991) Biomedical Research 12 (2): 97-103. 2. Kurobe, N., Suzuki, F., Okajima, K., and Kato, K. (1990) Clinica Chimica Acta 187: 11-20. 3. Kurobe, N., Inagaki, T., and Kato, K. (1990) Clinica Chimica Acta 192: 171-180. 4. Wong, G.H., Elwell, J.H., Oberley, L.W., and Goeddel, D.V. (1989) Cell 58: 923-931. 5. Manna, S.K., Zhang, H.J., Yan, T., Oberley, L.W., and Aggarwal, B.B. (1998) J. Biol. Chem. 273: 13245-13254. 6. Guegan, C. and Sola, B. (2000) Brain Res. 856: 93-100. 7. Zhang, H.J., Yan, T., Oberley, T.D. and Oberley, L.W. (1999) Cancer Res. 59: 6276-6283. 8. Ojika T., Imaizumi,M., Watanabe, H., Sakakibara, M., Abe, T., and Kato, K. (1991) Acta Histochem Cytochem 24(5): 489-495. 9. Shinder, G.A., Lacourse, M.-C., Minotti, S., Durham, H.D. (2001). J. Biol. Chem. 276(16): 12791-12796.