		Technical Data
S8060-09C	Superoxide Dismutase Cu,Zn (SOD)

Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:5000. Detects Cu/Zn SOD in RIPA lysates from A431 cells.
Optimal dilutions to be determined by the researcher.

Positive Antigen Control:
A0001-50 Non-stimulated A431 cell lysate. Add 2.5ul of 2- mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Use 10ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
250ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Recombinant protein corresponding to full-length human SOD (superoxide dismutase) plus an N-terminal methionyl.

Purity:

Serum

Form

Supplied as a liquid in 0.01M phosphate buffer, 0.1% sodium azide.

Specificity:

Recognizes human Cu/Zn SOD. MW: 16kD. Species Crossreactivity: Rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.