Technical Data
S8060-09C
Superoxide Dismutase Cu,Zn (SOD)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:5000. Detects Cu/Zn SOD in RIPA lysates from A431 cells.
Optimal dilutions to be determined by the researcher.

Positive Antigen Control:
A0001-50 Non-stimulated A431 cell lysate. Add 2.5ul of 2- mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Use 10ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant protein corresponding to full-length human SOD (superoxide dismutase) plus an N-terminal methionyl.
Purity:
Serum
Form
Supplied as a liquid in 0.01M phosphate buffer, 0.1% sodium azide.
Specificity:
Recognizes human Cu/Zn SOD. MW: 16kD. Species Crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.