Technical Data
S8060-09D
Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)
Description:
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparinbinding capacity (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
25ul4°C (-20°C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Rat Cu/Zn SOD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide, 50% glycerol.
Specificity:
Detects 23kD (human) and 19kD (other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots. Species Crossreactivity: rat, mouse, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Adachi, T, et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister, J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa,Y; O’Halloran, T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao, B, et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan, H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe, N, et al. (1990) Biomedical Research. 11: 187-194 7. Wispe, JR et al. (1989) BBA. 994: 30-36. 8. Xiao- Hong Lin, et al. (2001) J. Neurochem. 76: 1336-1345.