Technical Data
Superoxide Dismutase, Cu,Zn (SOD Cu,Zn)
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kD each are linked by two cysteines forming an intra-subunit disulfide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparinbinding capacity (1).

Suitable for use in Western Blot, Immunoprecipitation, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
25ul4°C (-20°C Glycerol)Blue IceRatRabbit
Rat Cu/Zn SOD
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.0, 0.09% sodium azide, 50% glycerol.
Detects 23kD (human) and 19kD (other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots. Species Crossreactivity: rat, mouse, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Adachi, T, et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister, J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa,Y; O’Halloran, T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao, B, et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan, H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe, N, et al. (1990) Biomedical Research. 11: 187-194 7. Wispe, JR et al. (1989) BBA. 994: 30-36. 8. Xiao- Hong Lin, et al. (2001) J. Neurochem. 76: 1336-1345.