Technical Data
S8060-10F
Superoxide Dismutase 2 (SOD2)
Description:
Superoxide dismutase (SOD) is an enzyme, which thought to play a role in the protection of aerobic cells against oxygen toxicity by catalyzing disputation of superoxide anion (O2-) to H2O2 and O2. SODs are found in 3 forms and produced by separate genes. The first isoforms (SOD1, also known as SOD-A, soluble SOD, Cytosolic SOD, Cu-Zn SOD and indophenoloxidase A or IPOA). Human SOD1 is 154 aa (chromosome 21q22). It is a homodimer and each subunit can bind 1 copper ion and 1 zinc ion. SOD1 is cytoplasmic protein. Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS) or amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Death usually occurs within 2 to 5 years. The familial form of ALS accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. SOD2 (mitochondrial indophenoloxidaeB, IPO-B, Mn-SOD) is a Mn-containing enzyme found primarily in mitochondria and therefore is not present in erythrocytes. SOD2 (human 222-aa; chromosome 6q25.3) is a homotetramer. It binds 1 Mn per subunit. SOD3 (extracellular-SOD or EC-SOD) is found in extracellular space (blood, lymph, synovial fluids and cerebrospinal fluid). Human SOD3 (240-aa, signal peptide 1-18aa; chromosome 4p15.3-p15.1) is a homotetramer.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1500; Use ECL technique.
ELISA: 1:500-1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4°C (-20°C Glycerol)Blue IceHumanSheep
Concentration:
~0.5mg/ml
Immunogen:
Highly purified SOD2 protein from human liver.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human SOD2 protein. No significant reactivity is seen with SOD1 or SOD3 proteins. Species Crossreactivity: Monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wispe JR et al (1989) BBA 994, 30-36; 2. Beck Y et al (1987) Nucl. Acid Res. 15, 9076; 3. Heckl K et al (1988) Nucl Acid Res. 16, 6224; 4. Ho YS et al (1988) FEBS Lett. 229, 25-260; 5. St Clair DK et al (1991) Cancer Res. 51, 939-943; 6. Church SL et al (1990) BBA 1087, 250-252; 7. Barra D et al (1984) JBC 12595-12601