Technical Data
S8060-10K
Superoxide Dismutase 2 (SOD2)
Description:
Superoxide dismutase comprises a family of metalloenzymes that catalyze the oxido-reduction of superoxide anion to H2O2. Manganese superoxide dismutase (SOD2) is encoded by nuclear chromatin, synthesized in the cytosol, and imported posttranslationally into the mitochondrial matrix (1). It has been shown that SOD2 activity in tumor cells is lower than that in their normal counterparts. results suggest that the reduced level of SOD2 activity observed in human tumor cells is not due to a defect in the primary structure of the SOD2 protein, a change in the dosage of the SOD2 gene, or a decrease in the stability of SOD2 mRNA in tumor cells but rather is due to a defect or defects in the expression of the gene (2). SOD2 is one of the major cellular defense enzymes that protects against toxic effects of superoxide radicals. Overexpression of human SOD2 has been shown to inhibit radiation-induced neoplastic transformation, suppress malignancy of cancer cells, and increase tolerance to various toxic agents (3).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E567Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human SOD2.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris- Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human SOD2 at ~25kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wispe JR, et al. Biochim Biophys Acta 994(1):30-6, 1989 2. St. Clair DK et al. Cancer Res 51(3):939-43, 1991 3. Wan XS, et al. DNA Cell Biol 13(11):1127-36