Technical Data
S8060-10N
Superoxide Dismutase 3 (Superoxide Dismutase 3 Extracellular, SOD 3, SOD3, Extracellular Superoxide Dismutase [Cu Zn], Extracellular Superoxide Dismutase Precursor, EC SOD, MGC20077)
Description:
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80kD. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Applications:
Suitable for use in Western Blot, Immunohistochemistryand Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1:1000
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10F772Affinity Purified
SizeStorageShippingSourceHost
25ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human EC-SOD purified from aortas.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide and 50% glycerol.
Specificity:
Recognizes EC-SOD. Species Crossreactivity: rat, other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Adachi T., et al. (1992) Clin Chim Acta. 212:89-102. 2. Barrister J.V., et al. (1987). Crit. Rev.
Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6.
4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491.