Technical Data
S8060-16H
Superoxide Dimutase (SOD) (HRP)
Description:
Antibody reacts with superoxide dismutase from bovine erythrocytes showing a 33kD band against this metallo-enzyme (Cu2+-Zn2+). The enzyme catalyzes the destruction of O2- and protects against harmful free radical effects. SOD is found in the CSF of patients with brain tumors although not readily detected in normal brain. Suitable for most immunological assays, including SOD quantitation in RBCs.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Fr) and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000.
Optimal dilutions to be determined by the researcher.

Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Storage and Stability:
Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
10mg-20°CBlue IceBovineRabbit
Concentration:
As reported
Immunogen:
Superoxide Dismutase [Bovine erythrocytes].
Purity:
Purified
Form
0.02M K Phosphate, 0.15M NaCl, gentamycin sulfate, pH7.2. Labeled with horseradish peroxidase (HRP).
Specificity:
Species Crossreactivity: Crossreacts with bovine. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981).