Technical Data
S8060-17B
Superoxide Dismutase 4 (SOD4)
Description:
Superoxide dismutase (SOD)is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu,Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu,Zn-SOD (EC-SOD/SOD-3). SOD1 isfound in all eukaryotic species as a homodimeric 32kD enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80kD tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to primary endogenous source of superoxide,the mitochondrial respiratory chain. SOD3 is aheparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways.SOD4 is a copper chaperone for superoxidedismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase.CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2,000
Immunoprecipitation: 2ul/400ul cell lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k10K358Ascites
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human protein purified from E.coli.
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human Superoxide Dismutase 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.