Technical Data
S8060-25E
Superoxide Dismutase, Mn (Manganese Superoxide Dismutase, Manganese SOD, Mn SOD, MnSOD, IPO-B, Superoxide Dismutase [Mn] Mitochondrial Precursor, Superoxide Dismutase 2 Mitochondrial, SOD 2, SOD2, SOD-2)
Description:
Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol. Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml. 1:2000.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Full length Superoxide Dismutase protein (Rat). Cellular Localization: Mitochondrial matrix.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide, 50% glycerol.
Specificity:
Recognizes rat Superoxide Dismutase. Detects a band at ~25kDa. Species Crossreactivity: human, mouse, Xenopus laevis, bovine, chicken, canine, Drosophilia melanogaster, guinea pig, hamster, monkey, porcine, rabbit, sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kurobe, N., Inagaki, T., Kato, K. (1990) Clinica Chimica Acta 192: 171-180.2. Kurobe, N., Suzuki, F., Okajima, K., Kato, K. (1990) Clinica Chimica Acta 187: 11-20.3. Wong, G.H., Elwell, J.H., Oberley, L.W., Goeddel, D.V. (1989) Cell 58: 923-931.4. Manna, S.K., Zhang, H.J., Yan, T., Oberley, L.W., Aggarwal, B.B. (1998) J. Biol. Chem. 273: 13245-13254. 5. Gonzalez-Zulueta, M., Ensz, L.M., Mukhina, G., Lebovitz, R.M., Zwacka, R.M., Engelhardt, J.F., Oberley, L.W., Dawson, V.L., Dawson, T.M. (1998) J. Neurosci 18: 2040-2055. 6. Li, Y., Huang, T.T., Carlson, E.J., Melov, S., Ursell, P.C., Olson, J.L., Noble, L.J., Yoshimura, M.P., Berger, C., Chan, P.H. (1995) Nat. Genet. 11: 376-381. 7. Ojika T., Imaizumi,M., Watanabe, H., Sakakibara, M., Abe, T., Kato, K. (1991) Acta Histochem Cytochem 24(5): 489-495. 8. Kurobe, N., Kato, K. (1991) Biomed. Rsch. 12: 97-103. 9. Xiao-Hong Liu, et al., (1993) Brain Res. 625: 29-37. 10. Bidmon, H-J., Kato, K., Schleicher, A., Witte, O.W., Zilles, K. (1998) Stroke 29: 203-211. 11. Hadjur, S., Ung, K., Wadsworth, L., Dimmick, J., Rajcan-Separovic, E., Scott, R.W., Buchwald, M., Jirik, F.R. (2001) Blood 98(4): 1003-1011. 12. Katsuki, H., Tomita, M., Takenaka, C., Shirakawa, H., Shimazu, S., Ibi, M., Kume, T., Kaneko, S., Akaike, A. (2001) J Neurochem 76(5): 1336-1345. 13. Guo, W., Adachi, T., Matsui, R., Xu, S., Jiang, B., Zou, M.H., Kirber, M., Lieberthal, W., Cohen, R.A. (2003) Am J Physiol 285(4): H1396-H1403.