Technical Data
S8060-33
Superoxide Dismutase 1 (SOD1)
Description:
Superoxide dismutase (SOD) is an enzyme, which thought to play a role in the protection of aerobic cells against oxygen toxicity by catalyzing disputation of superoxide anion (O2-) to H2O2 and O2. SODs are found in 3 forms and produced by separate genes. The first isoforms (SOD1, also known as SOD-A, soluble SOD, Cytosolic SOD, Cu-Zn SOD and indophenoloxidase A or IPOA). Human SOD1 is 154 aa (chromosome 21q22). It is a homodimer and each subunit can bind 1 copper ion and 1 zinc ion. SOD1 is cytoplasmic protein. Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS) or amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Death usually occurs within 2 to 5 years. The familial form of ALS accounts for about 10% of the cases and is transmitted in an autosomal dominant manner.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml (ECL).
ELISA: 0.1-1ug/ml; Control antigen can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17C47Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Highly purified SOD1 protein from human erythrocytes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes human SOD1. No significant reactivity is seen with SOD2 or SOD3 proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Barra D et al (1980) FEBS Lett. 12, 53-56. 2. Jabusch JR et al (1980) Biochem. 19, 2310-2316. 3. Levanin D et al (1985) EMBO J 4, 77-84. 4. Sherman L et al (1983) PNAS 80, 5465-5469.