		Technical Data
S8400	Surfactant Protein A (SP-A)

Description:
There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:4000-1:8000 with and without antigen retrieval. See Protocol.
Western Blot: 1:500. Detected glycosylated and unglycosylated isoforms of SP-A on 10ug of rat lung lysates; Band at ~26kD.
ELISA: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
I1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb
I1904-40E: IgG, H&L (Biotin) Pab Gt x Rb
I1904-46P: IgG, H&L (Biotin) Pab Gt x Rb
I1904-42T: IgG, H&L (Biotin) Pab Gt x Rb
I1904-42V: IgG, H&L (Biotin) Pab Gt x Rb

Recommended Control:
Lung tissue, lung lavage

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

Human SP-A (deglycosylated, beta-eliminated) purified from human alveolar proteinosis material.

Purity:

Serum

Form

Supplied as a liquid in PBS. No preservative added.

Specificity:

Reacts strongly with both glycosylated and unglycosylated isoforms of human SP-A at 26kD. Immunostains alveolar Type II cells, macrophages, and alveolar proteinosis material in both human proteinosis and in animal models of alveolar proteinosis. Species Crossreactivity: primate, rat and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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