Technical Data
S8401-35
Surfactant Protein B (Precursor)
Description:
Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kD) and SP-D (43kD) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kD) and SP-C (4kD) are noncollagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B (42-46kD) is glycosylated in the Golgi apparatus and undergoes carboxy-and aminoterminal proteolysis by a cathepsin D-like protease.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive control:
Lung adenocarcinomas.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3F338Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant pro-surfactant protein B. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S8401-35X
Specificity:
Recognizes human pro-SP-B at ~42-46kD. Does not recognize mature SP-B. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Khoor A, et. al. (1997) Mod Pathol, 10:6267