Technical Data
S8402
Surfactant Protein D, Lectin Domain (SP-D)
Description:
Surfactant Protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620kD under non-reducing conditions and 43kD under reducing conditions. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. SP-D is a lung surfactant associated collectin that has been implicated in the innate pulmonary host defense against a wide variety of important bacterial, fungal and viral pathogens.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Use snap frozen or paraffin embedded tissue): Fresh rat tissue: 1:400; fresh human tissue: 1:20. Fixation: Acetone 5 minutes at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: Microwave treatment 2 x 5 minutes at 750W for paraffin tissues.
ELISA: Use as a capture antibody.
Optimal dilutions to be determined by the researcher.

Source: Tissue culture supernatant

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2261Affinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceRatMouse
Concentration:
~0.4mg/ml
Immunogen:
Purified rat surfactant protein D from bronchoalveolar lavage.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from buffer, 5mg/ml BSA, 0.09% sodium azide. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells SP-A+ and SP-A- and extracellular deposits. Does not react with Alveolar type III cells. No reaction on rat skin, colon , kidney or liver. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Exp. Lung Res 21:577-588 (1995).