Technical Data
S8500-01N
Survivin (Apoptosis Inhibitor 4, Apoptosis Inhibitor-4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat-containing 5, BIRC5, BIRC-5, EPR1, EPR-1, IAP4, SVV, TIAP)
Description:
Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200 for 2 hours on human prostate cells
Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT. Requires boiling tissue in 10mM citrate buffer for 10-20 minutes followed by cooling at RT for 10-20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k9E764Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant full length human Survivin. Epitope: aa 1- 99. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
Specificity:
Recognizes human Survivin. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Geddis, A., et al. Blood. 104: 1017-1024, 2004. 2. Zeng SX, Li Y, Jin Y et al. Mol Cell Biol. 2009.