Technical Data
S8875-01E
SWAP70 (Switch-associated Protein 70, SWAP-70, HSPC321, KIAA0640)
Description:
SWAP70 is a 585aa regulatory protein belonging to the pleckstrin-like family with a PH domain essential for phosphatidylinositol 3,4,5-trisphosphate binding. SWAP70 acts as a link between the recognition of specific switch regions and causation of a DNA break and is involved in both nuclear events and in signaling B-cell activation. As a guanine nucleotide exchange factor (GEF) it transduces signals from tyrosine kinase receptors to RAC, independent of RAS. As a multiple functional signaling protein, and a component of the SWAP enzyme complex, it mediates signaling of membrane ruffling by tyrosine kinase growth factor receptors, actin cytoskeleton and cell protrusion. It is widely expressed in most tissues, mature B-cells, including those associated with mucosa-associated tissue and bronchus-associated tissue, monocytes and macrophages.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Daudi

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa300-350 of human SWAP-70.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human SWAP70. Species sequence homology: mouse, bovine, chimpanzee, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hilpela,P., Oberbanscheidt,P., Hahne,P., Hund,M., Kalhammer,G., Small,J.V. and Bahler,M. SWAP-70 identifies a transitional subset of actin filaments in motile cells. Mol. Biol. Cell 14 (8), 3242-3253 (2003). 2. Masat,L., Caldwell,J., Armstrong,R., Khoshnevisan,H., Jessberger,R., Herndier,B., Wabl,M. and Ferrick,D. Association of SWAP-70 with the B cell antigen receptor complex. Proc. Natl. Acad. Sci. U.S.A. 97 (5), 2180-2184 (2000). 3. Rapalus,L., Minegishi,Y., Lavoie,A., Cunningham-Rundles,C. and Conley,M.E. Analysis of SWAP-70 as a candidate gene for non-X-linked hyper IgM syndrome and common variable immunodeficiency. Clin. Immunol. 101 (3), 270-275 (2001).