Technical Data
Media
SMWTY Marine Medum (ATCC 231)
S9090
Microbiological Grade
Storage RT/4░C    Shipping RT
ATCC medium: 231 SWMTY - marine medium
Part A:
Potassium Nitrate0.5g
Sodium glycerophosphate0.1g
Trace Elements (see below)1.0ml
Tris buffer1.0g
Tryptone2.0g
Yeast extract2.0g
Trace Elements (Part B):
Boric Acid (H3BO3)2.85g
Cobalt ChlorideĽ6H2O40.4mg
Copper ChlorideĽ2H2O26.9mg
Ferric Sulfate1.36g
Manganese ChlorideĽ4H2O1.8g
Sodium MolybdateĽ2H2O25.2mg
Sodium tartrate1.77g
Seawater1.0L
Zinc Chloride20.8mg
Materials Needed But Not Supplied:
Agar15.0g
Seawater1.0L
Used for the cultivation and maintenance of a variety of heterotrophic marine bacterial species.


Directions per Liter: Dissolve 5.6 grams of Part A in 800-900ml of seawater stirring gently with heating until completely solubilized. Dissolve 7.9 grams of Part B in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring Part B to 1L. Take 1ml of Part B Solution and add to Part A Solution. Add additional water to Part A Solution to bring to 1L. Adjust to a final pH of 7.0. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121║C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Part A: Appearance

Solubility: Colorless to light yellow, clear, complete

pH: As Reported

Trace Elements (Part B): Appearance

Solubility: Colorless, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Handbook of Microbiological Media By Ronald M. Atlas