Technical Data
S9108-03A1
Synaptophysin (CT) (Major synaptic vesicle protein p38)
Description:
Synaptophysin is a protein of the presynaptic vesicle exocytosis machinery interacting at the vesicle with VAMP/synaptobrevin. Synaptophysin structure consists of a hexameric protein of four transmembrane spans with cytoplasmic amino and carboxyl termini. Although its actual function remains elusive, synaptophysin remains a specific presynaptic marker for all neurons, neuroendocrine cells and neuroderived neoplasms.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
PC12 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i216Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Synaptophysin.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Synaptophysin at and around the c-terminus. Species Crossreactivity: mouse, rat. Species sequence homology: bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.