Technical Data
S9108-03A2
Synaptophysin (CT) (Major synaptic vesicle protein p38)
Description:
Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa (1). It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for the presynaptic vesicle, and functions in synaptic vesicle exocytois, including cholesterol-dependent vesicle
biogeneis, regulation of SNARE assembly via interaction with VAMP/synaptobrevin, formation of the fusion pore initiating neurotransmitter release, and activation of vesicle endocytosis (2, 3). This gene has also been implicated in X linked mental retardation (4).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F773Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Immunoprecipitate of human brain.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.
Specificity:
Recognizes Immunoprecipitate of human brain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Johnston P.A., et al. (1989) J Biol Chem. 264:1268-1273. 2. Thiele C., et al. (2000) Nat Cell Biol. 2:42-49 3. Valtorta F., et al. (2004) Bioessays 26:445-453. 4. Tarpey P.S., et al. (2009) Nat Genet. 41(5):535-543.