Technical Data
S9108-05A
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.


Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the NSO mouse myeloma cell line.

Recommended Positive Control Tissue: Brain

Applications:
Immunohistochemistry: Paraffin. 1:500-1:1000
Western Blotting: 1:500
ELISA
Optimal working dilutions to be determined by researcher.

Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
TypeIsotypeCloneGrade
MabIgM3H2894Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Crude human synaptic immunoprecipitate.
Purity:
Purified by gel filtration.
Form
Purified IgM - liquid in PBS with 0.1% sodium azide.
Specificity:
3H2894 recognizes an antigen which appears to be identical to the human synaptic vesicle protein synaptophysin. It reacts with synaptophysin expressed in CHO cells (3., 8.). Species Crossreactivity: 3H2894 reacts with porcine, hamster and rat synaptophysin
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honer, W.G., Beach, T.G., Hu, L., Berry, K., Dorovini-Zis, K., Moore, G.R.W., Woodhurst, B. (1994). Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol. 87: 202-210.



2. Honer, W.G., Dickson, D.W.