Technical Data
S9108-05A
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:25. Requires heat induced antigen retrieval using citrate buffer pH 6.0.
Western Blot: 1:500
Optimal working dilutions to be determined by researcher.

Recommended Positive Control Tissue:
Pancreas

Hybridoma:
NSO Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM3H2894Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Crude human synaptic immunoprecipitate
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Specificity:
Recognizes human synaptophysin, also known as Major synaptic vesicle protein p38.. Species Crossreactivity: hamster, rat and marmoset
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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