Technical Data
S9108-06
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneths cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:40-1:80. Frozen & formalin/paraffin for 60 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Pancrea, rheochromocytoma, or thyroid.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG10.N.560Supernatant
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide encoding a region near the C-terminal end of the synaptophysin protein.
Purity:
Tissue culture supernatant
Form
Supplied as concentrated tissue culture supernatant in 0.09% sodium azide.
Specificity:
Recognizes human and rat Synaptophysin
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.