Technical Data
S9108-09
Synaptophysin (SYP, SYPH, SypI, Major Synaptic Vesicle Protein P38, Syn p38)
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.


Application(s):
Immunohistochemistry: For relatively cytoplasma-rich neuroendocrinic tumors a final concentration of 1ug/ml is recommended. For cytoplasma deficient tumors a concentration of 2ug/ml should be used.
TypeIsotypeCloneGrade
MabIgG12Q103Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Vesicular fraction of bovine brain.
Purity:
Purified immunoglobulin.
Form
Supplied as a liquid in PBS, pH 7.5, 5mg/ml BSA, 0.01% methylisothiazolone.
Specificity:
Recognizes bovine Synaptophysin. Reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with retina. Reacts with vesicles of adrenal medulla and islet cells and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. Stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12. Species crossreactivity: Human, rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wiedenmann, B. & Franke, W. W. Cell (1985) 41, 1017–1028. 2. Masliah, E., et al., PNAS.USA (2001) 98:12245–12250.