Technical Data
S9108-09B
Synaptophysin (SYP, SYPH, SypI, Major Synaptic Vesicle Protein P38, Syn p38)
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneths cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.


Applications:
Suitable for use in Immunoblot, Immunofluorescence and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material; Do not use protease pretreatment).

Recommended Dilution:
Immunohistochemistry: 1:50 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F304Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceBovineMouse
Concentration:
~0.05mg/ml
Immunogen:
Synaptophysin from presynaptic vesicles, prepared from bovine brain.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Specificity:
Represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: Mr 38,000 transmembrane glycoprotein of presynaptic vesicles. Reactivities on Cultured Cell Lines (tested so far): Rat PC-12 cell line. Species Crossreactivity: Human, bovine, rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gould, V.E., Wiedenmann, B., Lee, I., Schwechheimer, K., Dockhorn-Dworniczak, B., Radosevich, J.A., Moll, R. and Franke, W.W.: Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am. J. Pathol. 126, 243-257 (1987)
Gould, V.E., Lee, I., Wiedenmann, B., Moll, R., Chejfec, G. and Franke, W.W.: Synaptophysin: A novel marker for neurons, certain neuroendocrine cells and their neoplasms. Human Pathol. 17, 979-983 (1986)
Gould, V.E.: Synaptophysin - A new and promising Pan-neuroendocrine marker. Arch Pathol Lab Med 111, 791-794, (1987)
Kelly, R.B., Buckley, K.M., Burgess, T.L., Carlson, S.S., Caroni, P., Hooper, J.E., Katzen, A., Moore, H.-P., Pfeffer, S.R. and Schroer, T.A.: Membrane traffic in neurons and peptide-secreting cells. Cold Spring Harbor Symp. Quant. Biol. 48, 697-705 (1983)
Wiedenmann, B., Franke, W.W., Kuhn, C., Moll, R. and Gould, V.E.: Synaptophysin: A marker protein for euroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. USA 83, 3500-3504 (1986)
Wiedenmann, B. and Franke, W.W.: Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 45, 1017-1028 (1985)