Technical Data
S9108-09C
Synaptophysin (SYP, SYPH, SypI, Major Synaptic Vesicle Protein P38, Syn p38)
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (frozen and paraffin-embedded tissue): 1:10: Dilute with PBS, 1% BSA and 0.1% sodium azide. Incubation Time: 1hr at RT; extended for paraffin sections (overnight at 4°C). Proteolytic treatment is not recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
TypeIsotypeCloneGrade
MabIgG15F304Ascites
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceBovineMouse
Concentration:
Not Determined
Immunogen:
Synaptophysin from presynaptic vesicles, prepared from bovine brain.
Purity:
Ascites
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity:
Recognizes bovine Synaptophysin. Represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Reactivities on Cultured Cell Lines (tested so far): Rat PC-12 cell line. Species Crossreactivity: human, rat, and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gould, V.E., et al., Am. J. Pathol. 126, 243-257 (1987)Gould, V.E., et al., Human Pathol. 17, 979-983 (1986)Gould, V.E.: Arch Pathol Lab Med 111, 791-794, (1987)Kelly, R.B., Cold Spring Harbor Symp. Quant. Biol. 48, 697-705 (1983)Wiedenmann, B.,et all., Proc. Natl. Acad. Sci. USA 83, 3500-3504 (1986)Wiedenmann, B. and Franke, W.W.. Cell 45, 1017-1028 (1985)