This antiobody labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin embedded tissue and cytological material). Other applications not tested.
Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1hr at RT. Do NOT use protease pre-treatment.
Optimal dilution to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not determined|
|Synaptophysin from presynaptic vesicles, prepared from bovine brain.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4.|
|Excellent marker for several neuroendocrine tumors of epithelial origin (pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid) for neuronal and adrenal tumors (pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas). Reacts with transmembrane glycoprotein (Mr 38kD) in synaptic vesicles. Species reactivity: human, bovine, rat and mouse. Also reacts with rat PC-12 cell line.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Gould, V.E., Wiedenmann, B., Lee, I., Schwechheimer, K., Dockhorn-Dworniczak, B., Radosevich, J.A., Moll, R. and Franke, W.W.: Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am. J. Pathol. 126, 243-257 (1987)|
Gould, V.E., Lee, I., Wiedenmann, B., Moll, R., Chejfec, G. and Franke, W.W.: Synaptophysin: A novel marker for neurons, certain neuroendocrine cells and their neoplasms. Human Pathol. 17, 979-983 (1986)
Gould, V.E.: Synaptophysin - A new and promising Pan-neuroendocrine marker. Arch Pathol Lab Med 111, 791-794, (1987)
Kelly, R.B., Buckley, K.M., Burgess, T.L., Carlson, S.S., Caroni, P., Hooper, J.E., Katzen, A., Moore, H.-P., Pfeffer, S.R. and Schroer, T.A.: Membrane traffic in neurons and peptide-secreting cells. Cold Spring Harbor Symp. Quant. Biol. 48, 697-705 (1983)
Wiedenmann, B., Franke, W.W., Kuhn, C., Moll, R. and Gould, V.E.: Synaptophysin: A marker protein for euroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. USA 83, 3500-3504 (1986)
Wiedenmann, B. and Franke, W.W.: Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 45, 1017-1028 (1985)