Technical Data
S9108-13
Synaptophysin
Description:
Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas. ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas. Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:5000 on formalin-fixed human brain (hippocampus) using the Immunoperoxidase technique. Also stains paraffin embedded human tissue at 1:200 using the ABC technique.
Western Blot: 1:2500-1:10,000
ELISA: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM2Q107Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Crude human synaptic immunoprecipitate
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human Synaptophysin. Reacts with synaptophysin expressed in CHO cells. Species Crossreactivity: porcine, hamster, feline and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brain Research 609:9–20 (1993). Acta Neuropathol 87:135–142 (1994).