		Technical Data
S9108-15A	Synaptophysin, NT (Major Synaptic Vesicle Protein p38, SYP)

Description:
Synaptophysin is a transmembrane glycoprotein present in neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Coltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes, reminiscent of gap-junction proteins. Synaptophysin appears to be a significant cholesterol-binding protein in brain synaptic vesicles. A regioselective downregulation of synaptophysin is found in patients with Alzheimer.s disease and schizophrenia.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human Synaptophysin (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human Synaptophysin. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Yao, P.J., et al., Neurobiol. Aging 24(1):173-178 (2003). 2.Lietz, M., et al., Eur. J. Biochem. 270(1):2-9 (2003). 3.Kazantseva, I.A., et al., Arkh Patol 64(5):8-13 (2002). 4.Ulfig, N., et al., Neurosignals 11(2):95-102 (2002). 5.Fisher, S.E., et al., Genomics 45(2):340-347 (1997).