Technical Data
S9108-20
Synaptophysin
Description:
Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells(1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a
number of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is
preferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). Data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4).

Applications:
Suitable for use in: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:200,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Flow Cytometry: 1:20
Immunoprecipitation: 1:50
Optimal dilutions to be determined by researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG8k9Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in human Synaptophysin.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium azide, 0.05% BSA and 40% Glycerol.
Specificity:
Recognizes human Synaptophysin. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ozcelik T, et al. Am J Hum Genet 47(3):551-61, 1990. 2. McMahon HT, et al. Proc Natl Acad Sci 93(10):4760-4764, 1996. 3. Reisinger C, et al. J. Neurochem 90(1):1-8, 2004. 4. Tixier-Vidal A, et al. Neuroscience 26(3):847-61, 1988.