Technical Data
Synaptotagmin (SYT, DKFZp781D2042, P65, SVP65, Synaptotagmin I, SYT1, SYT-1, SytI, SYT2, SYT3)
Synaptotagmins are integral membrane proteins of synaptic vesicles that are thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
250ul-20CBlue IceMouseChicken
Four different affinity-purified anti-peptide antibodies were combined to make this product from human (NP_005630, NCBI) and mouse (NP_033332, NCBI) peptide shared sequences. The concentrations of each of the four antibodies was 100 ug/ml (based on Bradf
Purified by immunoaffinity chromatography.
PBS [pH7.2] containing 0.02% sodium azide
Species Crossreactivity: Reacts with mouse Synaptotagmin. Other species have not been tested. Homology with human is 100% for the immunizing peptides.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.