Technical Data
S9108-99
Synaptotagmin (Syt)
Description:
Synaptotagmins (Syt) are membrane-trafficking proteins characterized by an N-terminal transmembrane region, a variable linker, and two C-terminal C2-domains, C2A and C2B. At least twelve Synaptotagamins are expressed in vertebrates and can be found in both vesicular and plasma membranes.

Applications:
Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.

Recommended Dilutions:
Western Blot (Colorimetric): 1:1000
Optimal dilutions to be determined by researcher.

Recommended Positive Controls:
Mouse Brain Tissue Extract
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a4E208Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat brain synaptic junctional protein complexes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes a 65kD protein, corresponding to the apparent molecular mass of rat synaptotagmin. Species Crossreactivity: mouse and human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Matthew, W. D., Tsavaler, L. and Reichardt, L. F. (1981) J. Cell Biol., 91: 257-269 2. Baubet, V., Le Mouellic, H., Campbell, A.K., Lucas-Meunier, E., Fossier, P. and Brulet, P. (2000) PNAS 97: 7260-7265. 3. Sudhof, T.C. J Biol Chem (2002) J Biol Chem 277(10): 7629-7632. 4. Adolfsen, B., Littleton, J.T. (2001) Cell Mol Life Sci 58: 393-402.