Technical Data
S9109-19A
Synaptotagmin I (SYT1, DKFZp781D2042, p65, P65, SVP65, Synaptotagmin-1, SYT, SytI)
Description:
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain (hippocampus) and Mouse and Rat Brain lysates

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See S9109-19A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-RDLQSAEKEEQEK, from the internal region of human Synaptotagmin I.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Synaptotagmin I at ~65kD. Species sequence homology: mouse, rat, canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Martens S, Kozlov MM, McMahon HT. How synaptotagmin promotes membrane fusion. Science. 2007 May 25;316(5828):1205-8. Epub 2007 May 3