Technical Data
S9109-19F
Synaptotagmin 1 (Synaptotagmin-1, SYT1, Synaptotagmin I, SytI, P65)
Description:
Synaptotagmin is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release (Littleton and Bellen 1995). Recent studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane fusion (Zhang et al., 2002). It has recently been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size (Poskanzer et al., 2006).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat cortex lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to amino acid residues within the Synaptotagmin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug per ml BSA, 50% glycerol.
Specificity:
Recognizes rat Synaptotagmin. Species sequence homology: bovine, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Littleton JT, Bellen HJ (1995) Synaptotagmin controls and modulates synaptic vesicle fusion in a Ca(2+) dependent manner. Trends Neurosci. Apr;18(4):177-83. 2. Zhang X, Kim-Miller MJ, Fukuda M, Kowalchyk JA, Martin TF (2002) Ca2+ dependent synaptotagmin binding to SNAP25 is essential for Ca2+ triggered exocytosis. Neuron May 16;34(4):599-611. 3. Poskanzer KE, Fetter RD, Davis GW (2006) Discrete residues in the c(2)b domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Neuron Apr 6;50(1):49-62.