		Technical Data
S9109-19H	SYT1, ID (Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65, SYT)

Description:
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000
Western Blot: 0.3-1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide C-RDLQSAEKEEQEK from an internal region of human SYT1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human SYT1. Species sequence homology: bovine, canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.