Technical Data
S9109-24A
Synaptotagmin I/II (Synaptotagmin-1, SytI, p65, SYT1, SVP65, SYT, Synaptotagmin-2, SytII, SYT2)
Description:
The synaptotagmin family has been implicated in calcium-dependent neurotransmitter release, although Synaptotagmin I (Syt I) is the only isoform that controls synaptic vesicle fusion. Synaptotagmins have calcium-dependent membranetrafficking functions on both sides of the synapse. Synaptotagmins also play key roles in synaptic vesicle endocytosis. Synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission may be modulated by its phosphorylation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human Synaptotagmin I/II around the phosphorylation site of threonine 202/199 (R-K-TP-L-N).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Synaptotagmin I/II. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.