Technical Data
S9115-25
Synip (Syntaxin 4-Interacting protein)
Description:
Synip (Syntaxin 4-Interacting protein) specifically binds to Syntaxin 4 and is uniquely expressed in muscle and adipose tissue. The binding interaction of Synip with syntaxin 4 is regulated by insulin, which also induces dissociation of the Synip-syntaxin 4 complex. Synip plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane and functions as a positive regulator of insulin-stimulated GLUT4 translocation. Synip also competes with VAMP2 but Not SNAP23 for binding to Syntaxin 4 (1-3).

Applications:
Suitable for use in Flow Cytometry, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5,000-20,000 (70kD)
Immunohistochemistry: 1:250-500
Flow Cytometry: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k389Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near the C-terminus of the PDZ domain of human Synip.
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human Synip. Predicted to detect the isoform 2 base on sequence analysis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jeffrey E. Pessin, Debbie C. Thurmond, Jeffrey S. Elmendorf, Kenneth J. Coker, and Shuichi Okada, THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 274, No. 5, Issue of January 29, pp. 2593–2596, 1999
2. ing Min et al. Molecular Cell. Volume 3, Issue 6 , June 1999, Pages 751-760
3. J.A. Printen, M.J. Brady and A.R. Saltiel, Science 275 (1997), pp. 1475–1478

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