Technical Data
S9115-25
Synip (Syntaxin 4-Interacting protein)
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Technical Data |
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S9115-25 |
Synip (Syntaxin 4-Interacting protein) |
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Description: Synip (Syntaxin 4-Interacting protein) specifically binds to Syntaxin 4 and is uniquely expressed in muscle and adipose tissue. The binding interaction of Synip with syntaxin 4 is regulated by insulin, which also induces dissociation of the Synip-syntaxin 4 complex. Synip plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane and functions as a positive regulator of insulin-stimulated GLUT4 translocation. Synip also competes with VAMP2 but Not SNAP23 for binding to Syntaxin 4 (1-3). Applications: Suitable for use in Flow Cytometry, Western Blotting, Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5,000-20,000 (70kD) Immunohistochemistry: 1:250-500 Flow Cytometry: 1:40 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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