Technical Data
S9205-03Z
Syntaxin 1B (STX1B, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2, STX1B1, STX1B2)
Description:
Syntaxin 1B/STX1B is an ~33kD member of the Syntaxin family. It is a single-pass type IV transmembrane protein that is potentially involved in the docking of synaptic vesicles at presynaptic active zones. The cytoplasmic domain of Syntaxin 1B contains a coiled-coil Syntaxin domain aa29-180 which is implicated in the docking of synaptic vesicles with the presynaptic plasma membrane and a t-SNARE coiled coil domain aa191-253. Within the cytoplasmic domain, human Syntaxin 1B shares 100% aa sequence identity with mouse and rat Syntaxin 1B.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG112K181Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa2-264 from human Syntaxin 1B expressed in E.coli.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human Syntaxin 1B. Species sequence homology: Recombinant human Syntaxin 1A and 1B1. Does not react with rhSyntaxin 5, 6, 7, 8, 12, or 16.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.