Technical Data
S9205-25
Syntaxin 6 (Syntaxin-6, STX6, HGNC:11441)
Description:
Syntaxins are type II integral membrane proteins localized to membranes that regulate vesicle transport, protein maturation, and exocytosis. Syntaxin 1A resides in the nerve terminals of sensory neurons and nerve fibers reaching small blood vessels. Syntaxin 2 localizes to the apical plasma membrane and regulates epithelial-mesenchymal interactions. Syntaxin 6 is primarily localized to the trans-Golgi network, and partially co-localizes with AP-1 on clathrin-coated membranes. Syntaxin 13 is ubiquitously expressed and localizes to the tubular early and recycling endosomes, as well as endosomal vacuoles.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1ug/ml detects a band at ~35kD.
Immunoprecipitation: 12ug/ml
Optimal dilutions to be determined by researcher.

Positive Controls:
Mouse Brain Tissue Extract
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14E211Affinity Purified
SizeStorageShippingSourceHost
25ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat syntaxin 6. Epitope: amino terminus (aa1-25) of Syntaxin 6.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes rat Syntaxin 6. Species Crossreactivity: mouse, human, and hamster
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bock, J.B., Klumperman, J., Davanger, S. and Scheller, R.H. Mol. Cell Biol. 8: 1261-1271. (1997). 2. Bock, J.B., Lin, R.C. and Scheller, R.H. J. Biol. Chem. 271: 17961-17965. (1996). 3. Klumperman, J., Kuliawat, R., Griffith, J.M., Geuze, H.J. and Avran, P. J. Cell Biol. 141: 359-371. (1998). 4. Kimberly, W.T., LaVoie, M.J., Ostaszewski, B.L., Ye, W., Wolfe, M.S., Selkoe, D.J J Biol Chem 277(38): 35113-35117. (2002). 5. Fratti, R.A., Chua, J., Vergne, I., Deretic, V. Proc Natl Acad Sci USA 100(9): 5437-5442. (2003).